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KMID : 0869020060090010058
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Journal of Korean Orthopaedic Research Society
2006 Volume.9 No. 1 p.58 ~ p.64
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The Effect of Combined Immunosuppressive Treatment with FK 506-Cyclophosphamide on Surgical Angiogenesis
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Chung Yang-Guk
Kang Yong-Koo
Lee Yun-Kyung
Yang Young-Jun
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Abstract
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Purpose:To evaluate the effect of combined immunosuppressive treatment with FK 506-cyclophosphamide on surgical angiogenesis in non-vascularized bone xenografts.
Material and Methods:twenty-seven hamster-to-rat non-vascularized bone xenografts were performed. For surgical angiogenesis, recipient origin saphenous arteriovenous bundles were implanted into the medullary canals of graft bones. Immunosuppression with FK 506-cyclophosphamide was performed in group 1, not in group 2, control group. At postoperative 4 weeks, microangiography with microfil was conducted. Decalcified bones were cleared and the area of newly formed capillary, capillary density, were quantitatively evaluated using Scion image program. Bone specimens stained with hematoxylin-eosin were evaluated histologically also.
Results:There was no significant difference of capillary densities between immunosuppressed group and non-immunosuppressed control group (0.37+/-0.03/0.39+/-0.02, P=0.58). On histological examination, both groups showed neoangiogenesis into the medullary canals and endosteal bones.
Conclusion:FK 506-cyclophosphamide immunosuppression did not suppress the angiogenesis in non-vascularized bone xenografts, FK 506-cyclophosphamide combination of immunosuppression will be useful even in the condition of surgical angiogenesis.
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KEYWORD
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Non-vascularized bone xenograft, Surgical angiogenesis, FK 506-cyclophosphamide immuno-suppression, Capillary density
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